Description
The research group is focused on the optimization and biotechnological production of botanical and fungal biopesticides. We address the optimization of biopesticides by means of green chemistry processes on characterized extracts of plant and fungal origin already in production (artificial / biotech culture, field cultivation, fermentation) and agricultural waste and / or lead compounds.
Scientific objetives
- Preparation and characterization of botanical and fungal biopesticides
- Database and extract library: selective bioprospecting of plant and fungal endophytes. Screening their microextract activity against insect pests, nematodes and fungal pathogens. Metabolomic profiles. Identification of the active components and quantification of their activity.
- Optimization (chemical and biotechnological processes) of biopesticides: fungal biotransformations, biomimetic transformations and / or supercritical extraction.
- Biotechnological production: artificial-biotech crops (aeroponic, in vitro) of pre-selected plant species based on their bioactivity. Fermentation of endophytes producing active metabolites.
- Technology transfer: Plant Variety Registration and deposit of selected fungal strains. Patents (extracts / bioactive products, chemical processing and / or biotech formulations) in collaboration with other entities. Transfer of results through patent licensing options and / or trade secret.
Recent Highlights
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Plant genetic improvement against biotic stresses
Benzai, S.R.R., Giménez, R., Mignard, P. Mestre, L., Julián, C., González, V., Moreno, M.A. Biochemical characterization and differential expression of genes associated with apple tolerance to Penicillium expansum. Scientia Horticulturae 2024, 327:112853, https://doi.org/10.1016/j.scienta.2024.112853
Flores-León, A., García-Martínez, S., González, V., Garcés-Claver, A., Martín, R., Julián, C., Sifres, A., Pérez De Castro, A., Díez, M., López-Rincón, C., Ferriol. M., Gispert, C., Ruiz, J.J., Cebolla-Cornejo, J. & Picó, B. 2021. ”Grafting snake melon (Cucumis melo L. subsp. melo var. flexuosus (L.) Naudin) in organic farming: effects on agronomic performance, resistance to pathogens, sugars, acids and VOCs profiles, and consumer acceptance". Frontiers in Plant Science, https://doi.org/10.3389/fpls.2021.613845
Langa-Lomba, N., González-García, V., Martín-Ramos, P., Casanova-Gascón, J. “Screening of Vitis vinifera cultivars from the Grapevine Germplasm Bank of Aragon for susceptibility to Botryosphaeria dieback fungi”.Journal of Plant Diseases and Protection 2023. https://doi.org/10.1007/s41348-023-00741-9 -
Microbial ecology in agroecosystems
García-Barreda, S., Sánchez, S., Marco, P., Niccoló, G.M. & González, V. (2021). “Lack of linkages among fruiting depth, weight, and maturity in irrigated truffle fungi marks the complexity of relationships among morphogenetic stages”. Journal of Fungi, https://doi.org/10.3390/jof7020102
Mirás-Avalos, J.M., Marco, P. Sánchez, S. Bielsa, B. Rubio-Cabetas, M.J., González-García, V. “Soil Quality Index of Young and Differently Managed Almond Orchards under Mediterranean Conditions”. Sustainability, 2022, 14, 14770. https://doi.org/10.3390/su142214770
García-Barreda, S., Marco, P., Bonito, G., Parladé, J., Sánchez, S., González-García, V., Larena, I., Niccolo-Benucci, G.M. “Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests”. FEMS Microbiology Ecology 2023; https://doi.org/10.1093/femsec/fiad084
Langa-Lomba, N., Grimplet, J., Sánchez-Hernández, E., Martín-Ramos, P., Casanova-Gascón, J., Julián-Lagunas, C., González-García, V. “Metagenomic Study of Fungal Microbial Communities in Two PDO Somontano Vineyards (Huesca, Spain): Effects of Age, Plant Genotype, and Initial Phytosanitary Status on the Priming and Selection of their Associated Microorganisms”. Plants, 2023, 12(12), 2251; https://doi.org/10.3390/plants12122251 -
Integrated disease control
Langa-Lomba, N., Grimplet, J., Sánchez-Hernández, E., Martín-Ramos, P., Casanova-Gascón, J., Julián-Lagunas, C., González-García, V. “Metagenomic Study of Fungal Microbial Communities in Two PDO Somontano Vineyards (Huesca, Spain): Effects of Age, Plant Genotype, and Initial Phytosanitary Status on the Priming and Selection of their Associated Microorganisms”. Plants, 2023, 12(12), 2251; https://doi.org/10.3390/plants12122251
Sánchez-Hernández, E., González-García, V., Palacio-Bielsa, A., Lorenzo-Vidal, B., Buzón-Durán, L., Martín-Gil, J., Martín-Ramos, P. “Antibacterial Activity of Ginkgo biloba Leaf and Fruit Extracts against Clavibacter michiganensis subsp. michiganensis, Pseudomonas spp., and Xanthomonas vesicatoria”. Horticulturae 2023, 9(4), 461; https://doi.org/10.3390/horticulturae90404613
Sánchez-Hernández, Balduque-Gil, J., González-García, V., Barriuso-Vargas, J.J., Casanova-Gascón, Martín-Gil, J., Martín-Ramos, P. “Phytochemical Profiling of Aqueous Ammonia Extracts of Black Elder (Sambucus nigra L.) Flowers and Leaves and their Antifungal/Oomyceticidal Potential against Almond Tree Pathogens”. International Journal of Molecular Sciences 2023, 24(2), 1154; https://doi.org/10.3390/ijms24021154
N. Langa-Lomba; E.Sánchez-Hernández; L. Buzón-Durán; V. González-García; J. Casanova-Gascón; J. Martín-Gil; P. Martín-Ramos (2021). “Activity of anthracenediones and flavoring phenols in hydromethanolic extracts of Rubia tinctorum against grapevine phytopathogenic fungi”. Plants, https://doi.org/10.3390/plants10081527
N., Buzón-Durán, L., Sánchez-Hernández, E., Martín-Ramos, P., Casanova-Gascón, J., Martín-Gil, J., & González-García, V. (2021). “Antifungal Activity against Botryosphaeriaceae Fungi of the Hydro-Methanolic Extract of Silybum marianum Capitula Conjugated with Stevioside”. Plants, https://doi.org/10.3390/plants10071363z
González, V., Armijos, E. & Garcés-Claver, A. (2020). ”Fungal endophytes as biocontrol agents against the main soil-borne diseases of melon and watermelon in Spain”. Agronomy, https://doi.org/10.3390/agronomy10060820
Sánchez-Hernández, E., Balduque-Gil, J., Barriuso-Vargas, J.J., Casanova-Gascón, J., González-García, V., Cuchi-Oterino, J.A., Lorenzo-Vidal, B., Martín-Gil, J., Martín-Ramos, P. (2022). “Holm Oak (Quercus ilex subsp. ballota (Desf.) Samp.) Bark Aqueous Ammonia Extract for the Control of Invasive Forest Pathogens”. International Journal of Molecular Sciences, 23(19), 11882; https://doi.org/10.3390/ijms231911882
Langa-Lomba, N., Martín-Ramos, P., Casanova-Gascón, J., Julián-Lagunas, C., González-García, V. (2022). “Potential of Native Trichoderma Strains as Antagonists for the Control of Fungal Wood Pathologies in Young Grapevine Plants”. Agronomy 12(2), 336; https://doi.org/10.3390/agronomy12020336
Sánchez-Hernández, E., Martín-Ramos, P., Navas-Gracia, L.M., Martín-Gil, J., Garcés-Claver, A., Flores-León, A. & González-García, V. “Armeria maritima (Mill.) Willd. Flower Hydromethanolic Extract for Cucurbitaceae Fungal Diseases Control”. Molecules 2023, 28(9),
3730; https://doi.org/10.3390/molecules28093730 -
Characterization of the biocidal potential of essential oils and valorization of the active aqueous residues from their extraction
Sainz, P., Andrés, M. F., Martínez-Díaz, R. A., Bailén, M., Navarro-Rocha, J., Díaz, C. E., & González-Coloma, A. (2019). Chemical composition and biological activities of Artemisia pedemontana subsp. assoana essential oils and Hydrolate. Biomolecules, 9(10), 558. https://doi.org/10.3390/biom9100558
Navarro-Rocha, J., Andrés, M. F., Díaz, C. E., Burillo, J., & González-Coloma, A. (2020). Composition and biocidal properties of essential oil from pre-domesticated Spanish Satureja montana. Industrial crops and products, 145, 111958. https://doi.org/10.1016/j.indcrop.2019.111958
Valcarcel, F., Olmeda, A. S., Gonzalez, M. G., Andrés, M. F., Navarro-Rocha, J., & González-Coloma, A. (2021). Acaricidal and insect antifeedant effects of essential oils from selected aromatic plants and their main components. Frontiers in Agronomy, 3, 22. https://doi.org/10.3389/fagro.2021.662802
Soudani, S., Poza-Carrión, C., De la Cruz Gómez, N., González-Coloma, A., Andrés, M.F., Berrocal-Lobo, M. Essential Oils prime epigenetic and metabolomic changes for tomato defense against Fusarium oxysporum. Front. Plant Sci. 2022, 13. https://doi.org/10.3389/fpls.2022.804104
Ruiz-Vásquez, L.; Ruiz Mesia, L.; Caballero Ceferino, H.D.; Ruiz Mesia, W.; Andrés, M.F.; Díaz, C.E.; Gonzalez-Coloma, A. Antifungal and Herbicidal Potential of Piper Essential Oils from the Peruvian Amazonia. Plants 2022, 11, 1793.https://doi.org/10.3390/plants11141793
Galisteo, A.; González-Coloma, A.; Castillo, P.; Andrés, M.F. Valorization of the Hydrolate Byproduct from the Industrial Extraction of Purple Allium sativum Essential Oil as a Source of Nematicidal Products. Life 2022, 12, 905. https://doi.org/10.3390/life12060905
Kesraoui, S.; Andrés, M.F.; Berrocal-Lobo, M.; Soudani, S.; Gonzalez-Coloma, A. Direct and Indirect Effects of Essential Oils for Sustainable Crop Protection. Plants 2022, 11, 2144. https://doi.org/10.3390/plants11162144
Bailén M, Díaz-Castellanos I, Azami-Conesa I, Alonso Fernández S, Martínez-Díaz RA, Navarro-Rocha J, Gómez-Muñoz MT, González-Coloma A. Anti-Trichomonas gallinae activity of essential oils and main compounds from Lamiaceae and Asteraceae plants. Frontiers in Veterinary Science. 2022 ;9:981763. https://doi.org/1010.3389/fvets.2022.981763
JP Mbula, MF Andres, EM. Kitete, NG. Kasiama, DD. Tshilanda, KN. Ngbolua, DST Tshibangu, O Onautshu, A González-Coloma, PT Mpiana. Valorization of the essential oil from Drypetes gossweileri S. Moore (Putranjivaceae): in vitro, in vivo, and in silico nematicidal activity. Frontiers in Plant Science, 2023.
B Souda, MFAndres, W Elfalleh, A Gonzalez-Coloma, E Saadaoui (2024) GC-MS profiling, antifeedant, nematicidal and phytotoxic effects of essential oils of two subspecies of Eucalyptus flocktoniae (Maiden) Maiden, Natural Product Research, https://doi.org/10.1080/14786419.2023.2300392 -
Biopesticide activity of secondary metabolites produced by endophytic fungi
Morales-Sánchez, V., Fe Andrés, M., Díaz, C. E., & González-Coloma, A. (2020). Factors Affecting the Metabolite Productions in Endophytes: Biotechnological Approaches for Production of Metabolites. Current medicinal chemistry, 27(11), 1855-1873. https://doi.org/10.2174/0929867326666190626154421
Kaushik, N., Díaz, C. E., Chhipa, H., Julio, L. F., Andrés, M. F., & González-Coloma, A. (2020). Chemical composition of an Aphid antifeedant extract from an Endophytic Fungus, Trichoderma sp. EFI671. Microorganisms, 8(3), 420. https://doi.org/10.3390/microorganisms8030420
Morales-Sánchez, V., Díaz, C. E., Trujillo, E., Olmeda, S. A., Valcarcel, F., Muñoz, R.,... & González-Coloma, A. (2021). Bioactive metabolites from the Endophytic Fungus Aspergillus sp. SPH2. Journal of Fungi, 7(2), 109. https://doi.org/10.3390/jof7020109
Reyes Castillo N, Díaz CE, Andres MF, Imperial J, Valcárcel F, González-Coloma A*. Optimization of fungicidal and acaricidal metabolite production by endophytic fungus Aspergillus sp. SPH2. Bioresour. Bioprocess. 11, 28 (2024). https://doi.org/10.1186/s40643-024-00745-9
Diaz, C.E., Andres, M.F., Lacret, R. R Cabrera, C Gimenez, N Kaushik, A Gonzalez-Coloma*. Antifeedant, antifungal and nematicidal compounds from the endophyte Stemphylium solani isolated from wormwood. Sci Rep 14, 13500 (2024). https://doi.org/10.1038/s41598-024-64467-w
Díaz, CE*; Andrés, MF, Bolaños, P; González-Coloma, A*. Nematicidal and Insecticidal Compounds from the Laurel Forest Endophytic Fungus Phyllosticta sp. Molecules 2024, 29, 4568. https://doi.org/10.3390/molecules29194568 -
Synthesis and characterization of bioactive natural products
Galisteo Pretel, A., Pérez del Pulgar, H., Olmeda, A. S., Gonzalez-Coloma, A., Barrero, A. F., & Quílez del Moral, J. F. (2019). Novel insect antifeedant and ixodicidal nootkatone derivatives. Biomolecules, 9(11), 742. https://doi.org/10.3390/biom9110742
Galisteo Pretel, A., Pérez del Pulgar, H., Guerrero de León, E., López-Pérez, J. L., Olmeda, A. S., Gonzalez-Coloma, A.,... & Quílez del Moral, J. F. (2019). Germacrone derivatives as new insecticidal and acaricidal compounds: a structure-activity relationship. Molecules, 24(16), 2898. https://doi.org/10.3390/molecules24162898
Quílez del Moral, J. F., Pérez, Á., Navarro, M. J. S., Galisteo, A., Gonzalez-Coloma, A., Andrés, M. F., & Barrero, A. F. (2021). Selective Extraction of Bioactive Phenylethanoids from Digitalis obscura. Plants, 10(5), 959. https://doi.org/10.3390/plants10050959
González López, LA, Andres, MF, Quiñones, W, Echeverri, F, Gonzalez-Coloma, A*. (2024). Potential of 2-Hydroxyacetophenone Derivatives and Simple Phenols for the control of Meloidogyne javanica. Natural Product Communications. 19. https://doi.org/10.1177/1934578X241227689.
Torres-García, I.; Quílez del Moral, J.F.; Barrero, A.F.; González-Coloma, A.; Andrés, M.F.; López-Pérez, J.L.; Álvarez-Corral, M.; Rodríguez-García, I.; Muñoz-Dorado, M. Molecular Diversity from Longipinenes of Santolina viscosa Lag. through Acid Catalysis: Biocidal Activity. Biomolecules 2024, 14, 780.
M. J. Segura-Navarro, J.F. Quílez del Moral, A. Galisteo, J.L. López-Pérez, M.F.Andrés, A. González-Coloma, A.F. Barrero. Isolation and characterization of biopesticide terpenoids from Dittrichia viscosa (L.) roots. Phytochemistry, 2024
Galisteo, A., Zaher, H., Lopez-Menchero, J. R., Gonzalez-Coloma, A., Andres, M. F. de ., Moral, J. F. Q. del ., & Barrero, A. F. (2024, April). Valorization of the Sabinas Forest Waste (Juniperus phoenicea and Juniperus thurifera) as Source of Biopesticides. Natural Product Communications, 19(4). http://doi.org/10.1177/1934578x241232276