Description
The Metabolomics service: analysis of secondary metabolites, provides its services for the extraction and metabolomic analysis of botanical/fungal extracts, volatile and non-volatile.
The service has 2 scientists and 2 specialist technicians, with extensive experience, both in the extraction and obtaining of extracts, as well as in the analysis and interpretation of the results obtained from the metabolomic analysis of the extracts.
Benefits for extraction and obtaining extracts
- Soxhlet extraction using different sizes of Soxhlet bodies, depending on the quantity of source samples.
- Essential oil extraction by Clevenger.
- Organic extraction of aqueous samples, using different sizes of settling funnels, depending on the volume of the original sample.
Benefits for metabolomic analysis
- Metabolomic analysis of non-volatile extracts, using Liquid Chromatography coupled with mass spectrometry (HPLC-MS), performing both qualitative analysis of secondary metabolites and quantitative analysis with the desired product to be valued.
- Metabolomic analysis of volatile extracts, using Gas Chromatography coupled with mass spectrometry (GC-MS), performing both qualitative analysis of secondary metabolites and quantitative analysis with the desired product to be valued.
Equipment available for metabolomic analysis
- HPLC-MS 1
- Shimadzu LC-20AD pump.
- Shimadzu SPD-M20A Diode Array Detector.
- Shimadzu SIL-20A XR Auto Injector.
- Shimadzu LCMS-8040 mass spectrometer.
- HPLC-MS 2
- Dionex Ultimate 3000 RS pump.
- Detector Dionex Ultimate 3000 RS variable wavelenght detector.
- Dionex Ultimate 3000 RS autosampler.
- Bruker MS microTOF-QII mass spectrometer.
- GC-MS
- Shimadzu GC-2010 Gas Chromatograph.
- Shimadzu GCMS-QP2010 Ultra mass spectrometer.
- Shimadzu AOC-20i automatic injector.
- Libraries for product identification:
- Commercial libraries (Willey, NIST).
- The service's own natural products library.